SILENCING OF G-PROTEIN -SUBUNIT IN BARLEY (Hordeum vulgare L.) BY VIGS AND ITS EFFECT ON THE PLANT GROWTH

Authors : Muhammad Qasim SHAHID, Cuneyt UCARLI, Elif KARLIK, Semian Karaer UZUNER, Filiz GUREL

Pages : 259-264

Doi:10.17557/tjfc.75064

View : 64 | Download : 12

Publication Date : 2015-11-26

Article Type : Research Paper

Abstract :G-proteins play important roles in a number of cellular and developmental processes in plants. In this study, we have cloned a 317-bp fragment encoding a part of barley G protein -subunit into -genome of barley stripe mosaic virus insert ignore into journalissuearticles values(BSMV);. Barley seedlings were inoculated with this construct denoted as pSL038-1/G at 3-leaf stage, and assayed for their growth, leaf area and membrane ion leakage. Silencing was achieved after 2 weeks of inoculation and confirmed by the decrease in G subunit mRNAs in barley leaves. There was a significant decrease in plant growth in terms of plant height and leaf area following the viral infection. Leaf area in silenced plants were decreased by >10% compared to control plants insert ignore into journalissuearticles values(wild-type or inoculated by BSMV-PDS constructs);. In conclusion, silencing resulted in etiolated phenotypes and growth retardation. Our optimized VIGS protocol in barley could be adopted to evaluate extensive physiological parameters and molecular changes in G silenced plants.
Keywords : BSMV, G proteins, Ion Leakage, Phytoene desaturase PDS, Virus induced gene silencing VIGS,

ORIGINAL ARTICLE URL