IDENTIFICATION OF A SCAR MARKER LINKED TO A SHATTERING RESISTANCE TRAIT IN SESAME

Authors : Chalermpol PHUMICHAI, Weerachai MATTHAYATTHAWORN, Nipha CHUENPOM, Arunee WONGKAEW, Phakaked SOMSAENG, Tanapong YODYINGYONG, Pherawich PANKLANG, Sujin JENWEERAWAT, Yaowamarn KEAWSAARD, Thitaporn PHUMICHAI, Tanee SREEWONGCHAI, Rangsarid KAVEETA

Pages : 258-265

Doi:10.17557/tjfc.359707

View : 24 | Download : 10

Publication Date : 2017-12-15

Article Type : Research Paper

Abstract :Sesame insert ignore into journalissuearticles values(Sesamum indicum L.); is one of the most important oil crops in temperate and tropical regions and is grown worldwide over an area of 5179 insert ignore into journalissuearticles values(hg ha-1 ); to produce 5.469.024 tonnes of seed. Capsule shattering before or during harvest can cause yield losses of greater than 50%. The objectives of the present study were to evaluate the inheritance of resistance to capsule shattering in the F2 derived from a cross between Cplus1, a sesame line with shattering-resistant capsules insert ignore into journalissuearticles values(Sh1Sh1Sh2Sh2);, and KUAOX25, a line with shatteringsusceptible capsules insert ignore into journalissuearticles values(sh1sh1sh2sh2- );; and use bulked segregant analysis and Amplified Fragment Length Polymorphisms insert ignore into journalissuearticles values(AFLPs); to identify Sequence Characterized Amplified Region insert ignore into journalissuearticles values(SCAR); markers associated with shattering resistance. After screening 192 AFLP primer combinations, nine polymorphic bands were identified, and one of these AFLPs was developed into a Si-SR-32-19 SCAR that could distinguish between shattering-susceptible and shattering-resistant phenotypes.
Keywords : AFLP, BSA, SCAR, Sesamum indicum L, shattering resistance