IDENTIFICATION BY SSR AND SRAP MARKERS AND HETEROSIS ANALYSIS OF F1 HYBRIDS (Medicago ruthenica L.)

Authors : Yanting YANG, Rui GU, Jiaqi DONG, Yutong ZHANG, Fengling SHİ, Fang TANG

Pages : 163-169

Doi:10.17557/tjfc.1041042

View : 20 | Download : 7

Publication Date : 2021-12-27

Article Type : Research Paper

Abstract :Medicago ruthenica L. could be used as the crossbreeding material in forage crops to provide rich resistance gene resources based on its strong resistance to adversity stress. This study aimed to identify the authenticity and analyze heterosis of the intraspecific hybrids acquired from two Medicago ruthenica L. materials. The results showed 85 true hybrids in 118 F1 progenies identified by one SSR marker and five SRAP markers. Besides, the SRAP markers insert ignore into journalissuearticles values(13.89%); indicated higher identification efficiency than SSR markers insert ignore into journalissuearticles values(7.69%);, and the rate of true hybrids in HZ population insert ignore into journalissuearticles values(100%); was higher than that in ZH population insert ignore into journalissuearticles values(36.54%);. The six agronomic traits varied to different degrees, and their variation coefficients ranged from 18.53% to 45.72% in 13 hybrids of ZH population in 2019 and 2020. Moreover, ZH7 and ZH8 presented excellent agronomic performance, which could be used as candidate materials for further research. The mid-parent heterosis insert ignore into journalissuearticles values(Hm); of all agronomic traits was between -20.55% and 36.46%, and heterobeltiosis insert ignore into journalissuearticles values(Hh); showed negative values of 13 hybrids.
Keywords : Heterosis, intraspecific hybridization, Medicago ruthenica L, molecular marker