CYTOTOXIC EFFECT OF GREEN SYNTHESIZED SILVER NANOPARTICLES WITH SALVIA OFFICINALIS ON MCF-7 HUMAN BREAST CANCER CELLS

Authors : Şükriye YEŞİLOT, Soner DÖNMEZ

Pages : 133-139

View : 37 | Download : 13

Publication Date : 2021-12-27

Article Type : Research Paper

Abstract :Alternative in vitro nanotechnological methods have been developed to conventional methods in the treatment strategies of breast cancer. Silver nanoparticles insert ignore into journalissuearticles values(AgNPs); from metallic nanoparticles, especially those amplified using the green synthesis method, show promise as a suitable anticancer candidate in the field of nanomedicine. The purposed of the investigation was to green synthesize and characterize of silver nanoparticles insert ignore into journalissuearticles values(So-AgNPs); by Salvia officinalis aqueous extract and evaluate their anticancer effect on metastatic breast cancer cell line MCF-7. The formation of So-AgNPs were characterized by scanning electron microscopy insert ignore into journalissuearticles values(SEM); with Energy dispersive X-ray insert ignore into journalissuearticles values(EDX); and UV-visible spectroscopy. The surface absorption peak of So-AgNPs was observed at 417 nm by the UV-Vis analysis. MCF-7 and CRL-4010 cells were treated with different concentrations insert ignore into journalissuearticles values(0-0.05 µg/mL); of AgNPs for 24 h. The cytotoxic effect of green synthesized AgNPs against MCF-7 and CRL-4010 cell lines was approved by MTT [3-insert ignore into journalissuearticles values(4,5-dimethylthiazol-2- yl);-2,5-diphenyl tetrazolium bromide] assay. MTT results showed that So-AgNPs have higher cytotoxic activity in MCF-7 breast cancer cell lines than in CRL-4010 human breast epithelial cells insert ignore into journalissuearticles values(IC50 values 8.49 and 9.69 µg/mL, respectively);. AgNPs synthesized via Salvia officinalis extract exhibited a significant cytotoxic effect on MCF-7 cell lines, demonstrating that they could be a potential antitumor agent in the treatment of metastatic breast cancer. However, further research is required to elucidate the mechanisms of action.
Keywords : Siver nanoparticles, MFC 7, Salvia officinalis

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