Fraser Photinia shoot explantation in vitro: effects of two distinct gamma-ray sources and identification of the optimal mutation dose

Authors : Onur Sinan Türkmen, Kadriye Yaprak Kantoglu, Şeküre Şebnem Ellialtıoğlu

Pages : 13-19

View : 56 | Download : 28

Publication Date : 2025-06-29

Article Type : Research Paper

Abstract :Because of its beautiful qualities and ability to withstand harsh conditions, Fraser photinia (Photinia × fraseri cv. Red Robin) is frequently used as an ornamental plant in garden designs. The efforts to create new, highly marketable variations of the species have begun to increase in response to the growing ability of the current kinds to adapt to changing climatic circumstances. For this species, which is susceptible to in vitro propagation, the in vitro mutation breeding technique holds significant promise for increasing the current variety. It is essential to ascertain whether ionizing gamma ray sources are suitable for in vitro mutation investigations on Fraser photinia. In order to do this, in vitro shoot explants were exposed to a total of thirteen different doses of radiation using 60Co (dosage rate: 235 Gy/h) and 137Cs (dosage rate: 821 Gy/h) gamma ray sources. The number of leaves and shoot length in in vitro plantlets were assessed thirty days after irradiation, and linear regression analysis was used to get the EMD50 values. Based on the quantity of leaves, the EMD50 dose for the cesium-137 source ray application was 60.34 Gy, whereas the cobalt-60 source resulted in an EMD50 dose of 80.88 Gy. These findings demonstrated that the EMD50 dose difference was significantly impacted by the source power, irradiation duration, and the influence produced by the linear energy transfer value of the irradiation during tissue penetration.
Keywords : Fraser photinia, in vitro mutasyon, etkili mutasyon dozu

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