Pseudomonas aeruginosa Expressing Vitreoscilla Hemoglobin Shows Increased Production of L-Lysine α-Oxidase: an Enzyme used in Cancer Therapy

Authors : Hüseyin KAHRAMAN

Pages : 47-52

View : 23 | Download : 9

Publication Date : 2018-09-19

Article Type : Research Paper

Abstract :L-lysine α-oxidase insert ignore into journalissuearticles values(LO); is one of a few microbial enzymes with therapeutic potential in certain cancers. The enzyme has been determined in several bacteria and fungi. Its production is mainly regulated by carbon sources and oxygen. . Thus, the aim of this study was to determine the nutritional requirements and effect of oxygen concentration on the production of LO in P. aeruginosa , and in their recombinants using a highly efficient oxygen uptake system, the Vitreoscilla hemoglobin. This study concerns the effect of a higher oxygen uptake provided by a recombinant system, the Vitreoscilla hemoglobin, on the production of LO in Pseudomonas aeruginosa . The results showed that the recombinant bacterium expressing Vitreoscilla hemoglobin gene insert ignore into journalissuearticles values( vgb ); had distinct L-lysine activity from the host strain under both carbon catabolite repression and no repression conditions. In a rich medium supplemented with glucose, the recombinant strain showed 20-40 % higher L-lysine activity than the host strain. This difference was even more significant in the medium with no glucose supplement, where the recombinant strain showed almost 2-fold higher enzyme activity throughout the incubation. The results sometime were contradictory in terms of the effect of carbon source insert ignore into journalissuearticles values(mainly glucose); and oxygen on the production of this enzyme.
Keywords : Vitreoscilla hemoglobin, Psedomonas aeruginosa, L lysine α oxidase