Validation of stability-indicating high-performance liquid chromatography method for the determination of thymol in gelatin-based hydrogels

Authors : Onur Demir, Mehlika Pulat, Ali Bilgili

Pages : 132-139

Doi:10.51435/turkjac.1604250

View : 40 | Download : 64

Publication Date : 2025-05-31

Article Type : Research Paper

Abstract :The aim of this study was to develop an analytical method to determine the amount of thymol loaded into the structure of a gelatin hydrogel developed to struggle varroosis infestation of honey bees and released into a model release system. Chromatographic separation was achieved using a high-performance liquid chromatograph, C18 column and acetonitrile:water (75:25) mobile phase with an isocratic flow rate of 1 mL/min. To validate the method, specificity, precision, linearity, detection and measurement limit, accuracy, robustness, solution stability and system suitability parameters were studied. Hydrolytic, thermal, oxidative and photolytic degradation studies were performed for stress testing. The method is linear in the range of 0.25-15 μg/mL (R2 = 0.999). LOD was calculated as 6.2261 ng/mL and LOQ as 18.866 ng/mL. Intra-day and inter-day precision study %RSD values were ≤ 2%. The average recovery for the hydrogel was 100.3% (±2.12) and for the air sample was 99.9% (±3.14). The analytical method proved to be specific, linear, precise and robust. This study presents a sensitive, convenient and practical method for the detection of thymol in gelatin-based hydrogel structure and model release atmosphere.
Keywords : Kontrollü salım, HPLC, hidrojel, timol, validasyon, varroosis.

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