Comprehensive Phytochemical Profiling and Antioxidant Assessment of Rhinanthus serotinus subsp. aestivalis: GC-MS and HPLC-DAD Analysis of Volatile and Phenolic Compounds in Leaf and Flower Tissues

Authors : Abidin Gümrükçüoğlu

Pages : 347-360

Doi:10.51435/turkjac.1760137

View : 23 | Download : 42

Publication Date : 2025-09-30

Article Type : Research Paper

Abstract :This study represents the first comprehensive phytochemical characterization of Rhinanthus serotinus subsp. aestivalis, an understudied hemiparasitic species from the Orobanchaceae family. Using GC-MS and HPLC-DAD analyses, volatile and phenolic compound profiles were determined in leaf and flower extracts, along with antioxidant capacity evaluation via FRAP and CUPRAC assays. GC-MS analysis identified 16 volatile compounds, with matsutake alcohol (1-octen-3-ol) as the predominant constituent at exceptionally high concentrations (68.42% in flowers, 38.34% in leaves), significantly exceeding levels reported in related species. HPLC analysis revealed distinct tissue-specific phenolic profiles, with leaves demonstrating superior total phenolic content (7.26±0.28 mg GAE/g) and antioxidant capacity compared to flowers. Notable compounds included rutin (828.69 ppm), rosmarinic acid (594.16 ppm), and ascorbic acid (289.89 ppm) in leaves, while flowers were enriched in trans-caffeic acid (459.07 ppm) and pyrogallol (1062.84 ppm). These findings establish R. serotinus subsp. aestivalis as a promising source of bioactive compounds with potential applications in natural product development, functional foods, and pharmaceutical research, while providing fundamental data for future pharmacognostic studies.
Keywords : Rhinanthus serotinus, fitokimyasal analiz, matsutake alkolü, fenolik bileşikler, antioksidan aktivite, Orobanchaceae

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