Resveratrol’s Antiapoptotic and Antioxidant Role in Carbon Tetrachloride-Induced Nephrotoxicity of Rats

Authors : Feyza Başak, Güngör Çağdaş Dinçel, Betül Orhan, Serkan Yıldırım

Pages : 126-131

Doi:10.36483/vanvetj.1661888

View : 81 | Download : 75

Publication Date : 2025-07-27

Article Type : Research Paper

Abstract :This study investigates the protective effect of Resveratrol on renal tissues utilizing histopathology, immunohistochemistry, and immunofluorescence methods in a carbon tetrachloride (CCl4)-induced nephrotoxicity model. Wistar albinos (male, n=32) were randomly selected and divided into groups. The initial control group, followed by the second group, received CCl4 (2mg/kg), the third group was administered Resveratrol (30 mg/kg/day), and the fourth group was co-administered with CCl4 and Resveratrol. After the experiment, rats were euthanized under anesthesia, and their kidneys were collected. Results revealed that in the CCl4 group, notable degeneration and necrosis of tubular epithelium, significant steatosis, and arterial hyperemia wereobserved. In the CCl4+Resveratrol group, renal tissues exhibited slight deterioration of tubular epithelium and hyperemia in the glomerular arteries. The immunohistochemistry approach employing 8- Hydroxy-deoxyguanosine (8-OHdG) was utilized to identify DNA damage in kidney tissue. Immunohistochemistry results indicated that both the control and resveratrol groups had a negative reaction for 8-OHdG, but the CCl4 group demonstrated a statistically significant increase in 8-OHdG expression in the tubular epithelium located cytoplasmically (p<0.05). Modest cytoplasmic presence was detected in the CCl4+Resveratrol group. The immunofluorescence approach utilizing caspase 3 was employed to identify apoptosis in kidney tissue. The results indicated that the CCl4 group demonstrated a statistically significant increase in intracytoplasmic caspase 3 expression in the tubular epithelium compared to other groups (p<0.05) while a minor intracytoplasmic response for caspase 3 was observed in the CCl4+Resveratrol group. As a conclusion, resveratrol demonstrated strong antioxidant, antiapoptotic, and DNA-protective effects in a CCl-4-induced nephrotoxicity rat model and can be added to the daily diet for better kidney health against CCl-4-induced nephrotoxicity.
Keywords : Antioksidan, CCl4, Kaspaz 3, Resveratrol, 8-OHdG

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