Comparison of CLIA and ELFA Progesterone Assay Methods for Evaluating Pregnancy in Kilis Goats

Authors : Nurdan Coşkun, Fikret Karaca, Cafer Tayyar Ateş
Pages : 205-212
Doi:10.36483/vanvetj.1728594
View : 96 | Download : 241
Publication Date : 2025-11-30
Article Type : Research Paper
Abstract :Early pregnancy diagnosis is needed for small ruminants. Effective methods for early pregnancy detection are of great importance. Identifying pregnancy enhances rebreeding and is crucial for controlled breeding programs. However, various methods exist for diagnosing pregnancy in small ruminants. The choice of methods is influenced by equipment availability, the number of days post-insemination and the required accuracy level. Measurement of progesterone concentrations is used in pregnancy diagnosis in small ruminants. The detection of progesterone (P4) assays can facilitate the diagnosis of pregnancy approximately three weeks post-insemination. This study aimed to evaluate and compare the accuracy of two progesterone assays (CLIA and ELFA) for the early diagnosis of pregnancy (at day 21 post-insemination) in Kilis goats, using transrectal ultrasonography at day 50 as the reference standard. Kilis goats (n: 150) were synchronized with a 10-day intravaginal sponge. Artificial insemination applications were intracervically performed with frozen-thawed semen straws. Blood samples were collected 21 days post-insemination to assess progesterone levels for pregnancy diagnosis. The pregnancy rate and average progesterone level determined by the CLIA method were found to be higher than those determined by the ELFA method. Additionally, in early pregnancy determination, the number of truly pregnant animals was significantly higher, with sensitivity, specificity, and accuracy. The CLIA method is a precise and dependable approach for measuring plasma progesterone levels. In the study, it was concluded that the CLIA progesterone assay method evaluated for early pregnancy detection in Kilis goats was superior.
Keywords : CLIA, ELFA, Gebelik, Keçi, Progesteron seviyeleri

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