DETERMINATION OF THE EFFECT OF RUTIN ON EPITHHELIAL AND MESENCHIMAL TRANSITION IN PROSTATE CANCER CELLS

Authors : Esra BAL, Asuman DEVECİ ÖZKAN, Zeynep BETTS

Pages : 131-136

View : 35 | Download : 12

Publication Date : 2023-02-28

Article Type : Research Paper

Abstract :Objective: The difficulties experienced in the treatment of prostate cancer and the excess of side effects due to chemotherapy have brought the search for alternative treatment strategies. In recent studies, it is known that Rutin insert ignore into journalissuearticles values(RUT); has an anti-cancer effect on cancer cells. Our study aimed to determine the effects of RUT on epithelial-mesenchymal transition insert ignore into journalissuearticles values(EMT); in prostate cancer cells, for the first time. Methods: The anticancer activity of RUT in prostate cancer cells insert ignore into journalissuearticles values(PC-3); was determined by WST-1, Annexin V ELISA, DAPI and Acridine Orange staining, and the anti-cancer and anti-metastatic properties of RUT were evaluated with the Scratch Assay test. The mRNA expression level of Bax, Bcl-2, Snail, Twist, Vimentin and E-cadherin genes was determined by RT-PCR. Results: PC-3 cells were treated with RUT insert ignore into journalissuearticles values(500, 750, 1000, 1500 µM); for 24 and 48 hours. The viability rates decreased with increasing RUT concentration depending on dose and time insert ignore into journalissuearticles values(p<0.01);. Annexin V protein decreased, and apoptotic cells increased depending on the increasing RUT concentration insert ignore into journalissuearticles values(p<0.01);. There was a decrease in the number of migrating cells, especially at 750 and 1000 µM RUT concentrations. There was a significant increase insert ignore into journalissuearticles values(14.22-fold); in E-cadherin mRNA level after 1500 µM RUT treatment insert ignore into journalissuearticles values(p<0.01);. On the other hand, the mRNA expression level of Snail, Twist and Vimentin, decreased at higher RUT concentrations insert ignore into journalissuearticles values(p<0.01);. Conclusion: The existence of RUT\`s potential to inhibit epithelial-mesenchymal transition and promote apoptosis has been demonstrated. It is also recommended to investigate the effects of RUT on EMT in vivo.
Keywords : Prostat kanseri, rutin, epitelyal mezenkimal geçiş, apoptoz

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