Effect of ATP Addition to Goat Semen Post Thaw on Semen Parameters

Authors : Uğur Uçan, Zahid Naseer, Melih Aksoy

Pages : 46-51

Doi:10.53913/aduveterinary.1809432

View : 35 | Download : 107

Publication Date : 2025-12-25

Article Type : Research Paper

Abstract :In post-thaw goat spermatozoa the impact of ATP supplementation at different dose levels on motility and the other sperm parameters was evaluated during 4 hours of incubation. The ejaculate was frozen in 0.25ml straws by using egg yolk-glucose-glycerol-tris-citric acid extender. Thawing was achieved at 37°C for 30 second in a water bath and sperm cells were rediluted, following the removal of the extender, in tris-citric acid-glucose solution before adding 0 (control), 2, 4 or 8 mM of ATP. Eight of 10 parameters that were examined by the computer assisted sperm analyzer has been influenced by ATP supplementation (P 0.05) by ATP supplementation irrespective to the dose levels. As a result, ATP supplementation (2-4mM) to post-thaw goat spermatozoa improved progressive motility during the incubation (P<0.05) without any prominent influence on most of the kinematic sperm parameters including ALH, VCL, VAP and VSL. Further increase the ATP concentrations to 8mM suppressed motility parameters significantly (P<0.01). It was concluded that ATP supplementation might restore reduced motility problem, at least partly, in goat spermatozoa resulting from cryopreservation.
Keywords : akrozom reaksiyonu, ATP, Çözüm sonu, Teke sperması, motilite

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