In Vitro Antioxidant and Enzyme Inhibitory Activities of Walnut Male Flowers

Authors : Ebru AYDIN

Pages : 112-118

Doi:10.24323/akademik-gida.1350684

View : 54 | Download : 63

Publication Date : 2023-08-29

Article Type : Research Paper

Abstract :Walnut insert ignore into journalissuearticles values(Juglans regia L.); male flowers are known for their high phenolic content and associated health benefits, including anti-hypoxic, antihaemolytic, anti-inflammatory, antidepressant, and antioxidant activities. This study represents the first investigation of the inhibitory effects of walnut male flower extract on α-amylase and α-glucosidase enzymes, employing HPAE-PAD insert ignore into journalissuearticles values(High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection);. The inhibitory potential of the extract was compared to that of acarbose, a chemical drug commonly used for this purpose. Furthermore, the antioxidant activity of the extract was also evaluated. The extract demonstrated significant inhibition of α-amylase and α-glucosidase, with half maximal inhibitory concentration insert ignore into journalissuearticles values(IC50); values of 1.507 mg/mL and 0.803 mg/mL, respectively. In contrast, acarbose exhibited IC50 values of 1.031 mg/mL and 0.985 mg/mL for α-amylase and α-glucosidase, respectively. Although the walnut male flower showed greater inhibition of α-glucosidase than acarbose, acarbose exhibited stronger inhibition of α-amylase activity than walnut male flowers. The extract exhibited a DPPH insert ignore into journalissuearticles values(1,1-Diphenyl-2-picrylhydrazyl radical); free radical scavenging activity, with an IC50 value of 19.51 µg/mL. Additionally, the total phenolic content of 277 mg GAE insert ignore into journalissuearticles values(gallic acid equivalent);/g dry weight insert ignore into journalissuearticles values(dw); was determined in the extract. These results may highlight the potential of walnut male flowers as a novel enzyme inhibitor for managing type 2 diabetes mellitus. The findings of this study could provide valuable insights for further investigation into the potential applications of walnut male flowers in the food and pharmaceutical industries.
Keywords : Ceviz erkek çiçeği, α Amilaz, α Glukozidaz, Antioksidant, HPAE PAD