The changes in Alkaline, Neutral and Acid Protease Activities of ArtemiaEnriched with Commercial Emulsion and Different Additive Combinations

Mehmet NAZ; Gürkan DİKEN; Metin YAZICI; Yavuz MAZLUM; Selin SAYIN; Oktay SÖYLER

doi:10.26650/ASE2020793132

The changes in Alkaline, Neutral and Acid Protease Activities of ArtemiaEnriched with Commercial Emulsion and Different Additive Combinations

Authors : Mehmet NAZ, Gürkan DİKEN, Metin YAZICI, Yavuz MAZLUM, Selin SAYIN, Oktay SÖYLER

Pages : 152-158

Doi:10.26650/ASE2020793132

View : 31 | Download : 8

Publication Date : 2021-04-08

Article Type : Research Paper

Abstract :The biochemical compositions and the changes observed in alkaline, neutral and acid protease activities of Artemia enriched with commercial emulsion and different additive combinations were determined. Artemia nauplii insert ignore into journalissuearticles values(ArtN);, GroBiotic-A insert ignore into journalissuearticles values(GA);, Red Algamac insert ignore into journalissuearticles values(RA);, Red Algamac:GroBiot-ic-A insert ignore into journalissuearticles values(50:50); insert ignore into journalissuearticles values(RA:GA insert ignore into journalissuearticles values(50:50););, Spirulina insert ignore into journalissuearticles values(SP);, Spirulina:Red Algamac insert ignore into journalissuearticles values(50:50); insert ignore into journalissuearticles values(SP:RA insert ignore into journalissuearticles values(50:50););, Spiruli-na:GroBiotic-A insert ignore into journalissuearticles values(50:50); insert ignore into journalissuearticles values(SP:GA insert ignore into journalissuearticles values(50:50);); and Spirulina:Red Algamac:GroBiotic-A insert ignore into journalissuearticles values(33:33:33); insert ignore into journalissuearticles values(SP:RA:GA insert ignore into journalissuearticles values(33:33:33);); were tested in the study. The lowest and highest protein contents after the enrichment of Artemia were 40.74±1.02% insert ignore into journalissuearticles values(RA); and 55.03±1.26% insert ignore into journalissuearticles values(SP:RA:GA insert ignore into journalissuearticles values(33:33:33););, respectively. The lowest lipid contents of tested groups were found in 5.63±0.47% insert ignore into journalissuearticles values(GA); and 5.63±0.84% insert ignore into journalissuearticles values(RA:GA insert ignore into journalissuearticles values(50:50););. The highest lipid value after the enrichment were observed in 16.98±1.15 % insert ignore into journalissuearticles values(RA);. The lowest and highest ash values observed after the enrichment were 4.51±0.27% insert ignore into journalissuearticles values(SP:GA insert ignore into journalissuearticles values(50:50);); and 6.07±0.35% insert ignore into journalissuearticles values(RA); insert ignore into journalissuearticles values(p<0.05);. The lowest and highest protease activities of the pH=3, pH=4, pH=5, pH=6, pH=7 and pH=8.5 values were 18.18±0.37 U/mg protein insert ignore into journalissuearticles values(GA);, 31.04±0.38 U/mg protein insert ignore into journalissuearticles values(RA);, 9.1±0.32 U/mg protein insert ignore into journalissuearticles values(SP);, 9.66±0.19 U/mg protein insert ignore into journalissuearticles values(SP);, 16.94±0.61 U/mg protein insert ignore into journalissuearticles values(SP);, 63.09±0.75 U/mg protein insert ignore into journalissuearticles values(SP); and 33.77±0.59 U/mg protein insert ignore into journalissuearticles values(RA:GA insert ignore into journalissuearticles values(50:50););, 57,54±0.34 U/mg protein insert ignore into journalissuearticles values(RA:GA insert ignore into journalissuearticles values(50:50););, 23.75±0.28 U/mg protein insert ignore into journalissuearticles values(GA);, 40.82±0.49 U/mg protein insert ignore into journalissuearticles values(GA);, 69.94±0.65 U/mg protein insert ignore into journalissuearticles values(GA);, and 286.14±8.2 U/mg protein insert ignore into journalissuearticles values(GA); insert ignore into journalissuearticles values(p<0.05);. In conclusion, GA and SP:RA:GA insert ignore into journalissuearticles values(33:33:33); en-richment combinations are recommended as an alternative to enrichment products. On the other hand, SP and RA should not be used alone due to the disadvantages such as the biochemical composition and proteolytic enzyme activities of Artemia observed in the present study.
Keywords : Artemia, enrichment, GroBiotic A, biochemical compositions, enzymes

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