Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen

Authors : Münevver Müge ÇAĞAL, Ali Umman DOĞAN, Fatih ÇAKAR, Fikrettin ŞAHİN

Pages : 55-61

Doi:10.38042/biost.2020.29.02.01

View : 21 | Download : 10

Publication Date : 2020-12-30

Article Type : Research Paper

Abstract :In the present study, three entomopathogenic strains of Bacillus sphaericus insert ignore into journalissuearticles values(MBI5, MBI6, and MBI7); were isolated from greenhouse pest and larval habitat of Istanbul, Turkey. All of the MBI groups tested in laboratory bioassays were able to kill larvae, but not pupae and adults of Culex spp. in water. The most efficient larvicidal activity of the strains was found in the presence of two toxin genes, binA and binB for MBI5, MBI6, and MBI7 strains. Each B. sphaericus strains identified in this study were characterized as unique and novel in terms of fatty acid methyl ester insert ignore into journalissuearticles values(FAME); profiles and 16S rRNA sequencing data. The results of this study suggest that the three strains of B. sphaericus may be new sources of potential biocontrol agent of mosquito.
Keywords : Bacillus sphaericus, Mosquito, Biocontrol