Effects of calcium concentration, calcium chelators, calcium channel-blockers on Hsp70a expression in Chlamydomonas reinhardtii

Authors : Tuba SEVGİ, Elif DEMİRKAN

Pages : 10-16

Doi:10.38042/biotechstudies.1069555

View : 17 | Download : 9

Publication Date : 2022-06-15

Article Type : Research Paper

Abstract :In this study, calcium concentration, calcium chelators, and calcium channel blockers that could be effective in triggering the heat shock response in Chlamydomonas reinhardtii were investigated. For this purpose, continuously expressed and heat-inducible transformant C. reinhardtii strains were used, and heterologously expressed arylsulfatase activities were detected. After a short time of heat shock at 40°C, cultures were shifted to 23°C and different concentrations of calcium insert ignore into journalissuearticles values(0-1 M CaCl2);, EGTA insert ignore into journalissuearticles values(0-50 mM);, BAPTA insert ignore into journalissuearticles values(0-2 mM);, lanthanum insert ignore into journalissuearticles values(0-300 µM);, gadolinium insert ignore into journalissuearticles values(0-350 µM);, and verapamil insert ignore into journalissuearticles values(0-100 µM); applications were performed. To compare the arylsulfatase activity results at the transcript level, HSP70A expression level was analyzed. Arylsulfatase activity was increased with the increase of the calcium concentration, in the presence of calcium chelators, blockers, and parallel results were obtained in HSP70A expression level. These findings support that both extracellular and intracellular calcium influx is effective in the heat shock response of C. reinhardtii.
Keywords : Arylsulfatase, Heat shock response, Membrane fluidity