qPCR analysis of Puccinia spp. in turfgrass areas in Türkiye

Authors : Filiz Ünal, Senem Tülek

Pages : 66-70

Doi:10.16955/bitkorb.1365353

View : 139 | Download : 146

Publication Date : 2023-12-31

Article Type : Research Paper

Abstract :In recent years, with the increase in parks, gardens, recreation areas, picnic areas and refuges with urbanization, the interest in turfgrass areas that beautify the aesthetic appearance of these areas has also increased. In these areas, rust diseases are also becoming increasingly common and causing problems. Rust diseases are an important pathogen group that needs to be monitored due to their ability to create new races and their airborne transmission. In this study, surveys were conducted in turfgrass areas in 8 provinces of Türkiye and 110 turfgrass leaf samples showing rust symptoms were collected. As a result of DNA isolation and qPCR analysis from pustules on the leaf surface, 37 Puccinia coronata, 32 P. graminis, 18 P. striiformis and 23 Puccinia spp. have been detected. It was determined that the most common rust species that causes disease in turfgrass areas in Türkiye is P. coronata. This species was followed by P. graminis and P. striiformis, respectively. While P. striiformis was mostly isolated from Kayseri and Istanbul provinces, P. coronata and P. graminis were mostly isolated from İstanbul.
Keywords : çim, pas, real time PCR, Türkiye