Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes

Authors : Turgut TAŞKIN, Tugce YESİL DEVECİOGLU, İsmail ŞENKARDEŞ

Pages : 190-195

Doi:10.5152/clinexphealthsci.2017.651

View : 23 | Download : 10

Publication Date : 2018-09-28

Article Type : Research Paper

Abstract :Objective: Terfezia claveryi is a dark brown color truffle and has been used extensively in human diet in Turkey. Therefore; it is an important issue to determine their biological activities and toxicity. This study aims to investigate the antioxidant and anti-urease effects of T. claveryi methanol extracts and to evaluate the genotoxicity of each extract. Methods: The antioxidant activities of methanol extracts were examined by 2,2-diphenyl-1-picryl-hydrazyl insert ignore into journalissuearticles values(DPPH);, 2,2’-azino-bisinsert ignore into journalissuearticles values(3-ethylbenzothiazoline-6- sulfonate insert ignore into journalissuearticles values(ABTS); and ferric reducing/antioxidant power insert ignore into journalissuearticles values(FRAP); methods. The amount of total phenolic compounds contained in the extracts was determined using the Folin-Ciocalteu reagent insert ignore into journalissuearticles values(FCR); method. Anti-urease activities of methanol extracts were evaluated by indophenol method. In addition, potential DNA damaging effects of T. claveryi extracts on human lymphocytes were determined by the alkaline comet assay. Results: UM extract exhibited higher DPPH radical scavenging activity than MM extract [the concentration of the substance which provides the inhibition of 50% of the radical insert ignore into journalissuearticles values(IC 50 );:0.77±0.01 mg/mL, 1.17±0.02 mg/mL, respectively, p <0.0001] however, lower activity than standard insert ignore into journalissuearticles values( p <0.0001);. Both extracts in different concentrations of T. claveryi caused DNA damage insert ignore into journalissuearticles values( p <0.0001); compared with solvent control DMSO. Also, total comet scores was higher in cells which had been incubated with MM extract insert ignore into journalissuearticles values( p <0.05);. UM extract exhibited higher ferric reducing and anti-urease activity than MM extract but not found statistically different; also activities of both extract were found lower than positive controls of each methods insert ignore into journalissuearticles values( p <0.0001);. Conclusion: T. claveryi methanol extracts induced genotoxicity on lymphocytes and showed very low antioxidant activity compared to standards. This preliminary study should be confirmed with further studies and in diferent cell lines.
Keywords : T claveryi, genotoxic, antioxidant, anti urease, maceration, ultrasonic bath