Investigation of the Effects of Biological Activity and Steeping Time of Four Different Black Teas Commercially Valuable in Turkey

Authors : Turgut TASKİN, Muhammed LEVENT, Talip SAHİN

Pages : 283-287

Doi:10.33808/clinexphealthsci.516099

View : 25 | Download : 10

Publication Date : 2019-12-31

Article Type : Research Paper

Abstract :Objective: The aim of this study was to compare the phenolic, tannins contents and biological activities insert ignore into journalissuearticles values(antioxidant, anticholinesterase); of four different black teas commercially valuable in Turkey. Subsequently, to examine the effect of the steeping time on the chemical contents insert ignore into journalissuearticles values(phenolic and tannins contents); and biological activities of the samples. Methods: The antioxidant activities of samples were examined by 2,2-diphenyl-1-picryl-hydrazyl insert ignore into journalissuearticles values(DPPH);, cupric reducing antioxidant capacity insert ignore into journalissuearticles values(CUPRAC); and ferric reducing/antioxidant power insert ignore into journalissuearticles values(FRAP); methods. The amount of total phenolic and tannins contents contained in the samples was determined using the Folin-Ciocalteu reagent insert ignore into journalissuearticles values(FCR); method. In addition, the anticholinesterase activities of different samples were evaluated by Ellman method. Results: According to the results obtained, filtered tea bag insert ignore into journalissuearticles values(FT); sample obtained after 5 min steeping exhibited stronger DPPH radical scavenging activity than other samples. In addition, it was found that powder ceylon tea insert ignore into journalissuearticles values(PCT); samples obtained after 60 min and 10 min steeping had the highest FRAP and CUPRAC values respectively. In this study, it was also determined that the PCT samples obtained after 5 min, 10 min, 15 min, and 30 min steeping contained higher phenolic and tannins contents than the other samples. In anticholinesterase experiment, powder tea insert ignore into journalissuearticles values(PT); sample obtained after 5 min steeping was found to have the strongest anticholinesterase activity. Conclusion: As a result, it was determined that the amount of phenolic contents generally increased with the infusion time. It was also found that the antioxidant, anticholinesterase activities and tannins content of the tea samples did not show significant change due to the time of the infusion.
Keywords : Black teas, antioxidant, anticholinesterase, steeping time