Determination of Rank, Rankl and Opg Gene Polymorphisms in Triple-Negative Breast Cancer Patients and Invesgation of its Effect on Bone Metastasis

Authors : Şükran YILDIZ, Hani AL SAADONI, Mehmet ALIUSTAOGLU, Arzu ERGEN, Sadrettin PENCE

Pages : 316-320

Doi:10.33808/clinexphealthsci.533548

View : 23 | Download : 10

Publication Date : 2019-12-31

Article Type : Research Paper

Abstract :Objective: Triple negative breast cancer insert ignore into journalissuearticles values(TNBC); is a sub-type of breast cancer with the worst prognosis and highest risk of mortality. Bone metastasis is the most common metastasis type among women with breast cancer. RANK and OPG, are the members of the family of tumor necrosis factor insert ignore into journalissuearticles values(TNF);, which is effective on osteoblastic and osteoclastic mechanisms. RANKL, interacts with RANK and leads to bone resorption, whereas it inhibits bone destruction when it interacts with OPG. Methods: In this study, we investigated the polymorphisms of RANK, RANKL and OPG genes and their effects on bone metastasis in 45 patients with triple negative breast cancer and 30 healthy controls, using PCR, RFLP and agarose gel electrophoresis techniques. Results: The RANKL genotype and allele distribution analysis revealed a significantly increased CC genotype incidence in patients with TNBC and bone metastasis insert ignore into journalissuearticles values(p=0.011); and in those without bone metastasis insert ignore into journalissuearticles values(p=0.004); compared to the control group. The OPG genotype and allele distribution analysis revealed significantly increased C allele incidence in patients with TNBC and bone metastasis insert ignore into journalissuearticles values(p=0.004); compared to the control group. Likewise, the CC genotype insert ignore into journalissuearticles values(p=0.001); and C allele incidences insert ignore into journalissuearticles values(p=0.001); were observed to be significantly increased in patients with TNBC compared to healthy controls. Conclusion: This study is one of the first studies investigating all three RANK/RANKL/OPG gene polymorphisms and the relationship between breast cancer and bone metastasis in our country. We believe that our study will shed light onto further studies to be conducted on triple negative breast cancer and bone metastasis.
Keywords : Meme, Metastaz