Alterations in Enzyme Activity of Carbonic Anhydrase, 6-phosphogluconate Dehydrogenase and Thioredoxin Reductase in Rats Exposed to Doxorubicin and Morin

Authors : Ahmet Gökhan AĞGÜL, Müslüm KUZU, Fatih Mehmet KANDEMİR, Sefa KÜÇÜKLER, Cüneyt ÇAĞLAYAN

Pages : 228-234

Doi:10.33808/clinexphealthsci.632320

View : 26 | Download : 9

Publication Date : 2020-09-29

Article Type : Research Paper

Abstract :Objective: Carbonic anhydrase insert ignore into journalissuearticles values(CA);, 6-phosphogluconate dehydrogenase insert ignore into journalissuearticles values(6PGD); and thioredoxin reductase insert ignore into journalissuearticles values(TrxR); enzymes are the essential biological molecules needed for metabolic processes in all living cells. This study was designed to investigate the activities of CA, 6PGD and TrxR enzymes in the brain, kidney, liver, heart and testis tissues of the rats exposed to Dox and morin. Methods: Male Wistar albino rats were randomly divided into three groups as control, morin and DOX, each of them containing 7 rats. At the end of the experimental procedure, CA, 6PGD and TrxR enzyme activities in tissues of rats were determined spectrophotometrically. Results: In our study, we observed that DOX activated CA enzyme in liver and kidney tissues while inhibiting CA enzyme in the other tissues, activated 6PGD enzyme in the kidney, liver and heart tissues, and inhibited the TrxR enzyme in all the tissues. In addition, morin activated CA enzyme in the liver tissue while inhibiting CA enzyme in the brain, heart and testis tissues. Morin activated 6PGD enzyme activity while it inhibited TrxR enzyme in all the tissues. Conclusion: The findings showed that doxorubicin and morin had similar properties in the tissues as to their effect on enzyme activities.
Keywords : Doxorubicin, Morin, Carbonic Anhydrase, 6 phosphogluconate Dehydrogenase, Thioredoxin Reductase