Investigation of Neurogenesis in Kindled Wistar and Genetic Absence Epilepsy Rats

Authors : Cansu KANDEMİR, Melis YAVUZ, Fatma Bedia KARAKAYA, Özlem Tuğçe ÇİLİNGİRKAYA, Filiz ONAT, Serap ŞİRVANCI

Pages : 753-759

Doi:10.33808/clinexphealthsci.1021171

View : 22 | Download : 13

Publication Date : 2022-09-28

Article Type : Research Paper

Abstract :Objective: The most common type of epilepsy affecting about 50 million people worldwide is temporal lobe epilepsy insert ignore into journalissuearticles values(TLE);. Chemical and electrical kindling methods in animals can be used to form TLE model. In the present study, it was aimed to investigate neurogenesis in the hippocampus of adult kindled Wistar rats and genetic absence epilepsy rats from Strasbourg insert ignore into journalissuearticles values(GAERS); rats by immunofluorescence methods. Methods: Adult Wistar and GAERS albino rats weighing 250-300 gr were injected pentylenetetrazole insert ignore into journalissuearticles values(PTZ); insert ignore into journalissuearticles values(35 mg/kg, s.c.); every other day to produce chemical kindling. Animals having 5 times grade 5 seizures were considered to be kindled. Intracardiac perfusion was performed under deep anesthesia on the 7th and 14th days after the last grade 5 seizure. Immunofluorescence methods were used to demonstrate newly formed neurons, astroglial cells, and mature neurons, by using anti-doublecortin insert ignore into journalissuearticles values(DCX);, anti-glial fibrillary acidic protein insert ignore into journalissuearticles values(GFAP);, and anti- neuronal nuclear antigen insert ignore into journalissuearticles values(NeuN); primary antibodies, respectively. Sections were then examined under a fluorescence microscope. Results: DCX insert ignore into journalissuearticles values(+); cells were found to be increased in GAERS control groups compared to the Wistar control groups; and in Wistar PTZ groups compared to the Wistar control groups. DCX insert ignore into journalissuearticles values(+); cells were decreased in GAERS PTZ groups compared to their controls and to Wistar PTZ groups. Conclusion: The findings of the present study suggest that the resistance to electrical kindling of GAERS reported in previous studies might be related to the increased neurogenesis in this strain.
Keywords : Doublecortin, hippocampus, kindling, neurogenesis