The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats

Authors : Ahmet BAKIR, Suat EKİN, Sevgi YÜKSEK, Gokhan OTO

Pages : 587-593

Doi:10.33808/clinexphealthsci.943255

View : 26 | Download : 9

Publication Date : 2022-09-28

Article Type : Research Paper

Abstract :Objective: This study was designed to examine the potential protective effects of Rheum ribes L., and quercetin on protein carbonyl insert ignore into journalissuearticles values(PCO); in kidney and liver tissue, trace elements insert ignore into journalissuearticles values(Fe, Cu, Zn); and mineral insert ignore into journalissuearticles values(P); in serum samples in Wistar rats of carbon tetrachloride insert ignore into journalissuearticles values(CCl4);-induced oxidative damage. Methods: The 2, 4-dinitrophenylhydrazine insert ignore into journalissuearticles values(DNPH); method is the most reliable method widely used to measure carbonyl levels in proteins. In this study, the effect of Rheum ribes L. insert ignore into journalissuearticles values(Rr); and quercetin on protein carbonyl, trace elements insert ignore into journalissuearticles values(Fe, Cu, Zn); and mineral insert ignore into journalissuearticles values(P); levels against carbon tetrachloride insert ignore into journalissuearticles values(CCl4); mediated liver and kidney damage was investigated. For this purpose, 56 Wistar albino female rats weighing 200 ± 220 g were used. Groups were designed as: controls, 0.3 ml DMSO, 1 ml/kg olive oil, 1 ml/kg CCl4, 100 mg/kg Rr, 100 mg/kg quercetin, 100 mg/kg Rr+1 ml/kg CCl4 and 100 mg/kg quercetin+1 ml/kg CCl4 groups. Results: The results showed that the CCl4 group had significantly higher level of protein carbonyl insert ignore into journalissuearticles values(PCO); than the control, DMSO, olive oil, Rr and quercetin groups insert ignore into journalissuearticles values(p<0.001, p<0.05, p<0.001, p<0.01, and p<0.01, respectively);. A significant elevation in the group of CCl4 + quercetin, compare to control, DMSO, olive oil, Rr and quercetin groups insert ignore into journalissuearticles values(p<0.001, p<0.05, p<0.001, p<0.01, and p<0.05, respectively); in the liver tissue. Additionally, the CCl4 group had significantly higher level of PCO than the control, DMSO, olive oil, Rr and quercetin groups insert ignore into journalissuearticles values(p<0.001, p<0.01, p<0.001, p<0.001, and p<0.001, respectively);. Similarly, the CCl4 + Quercetin group had increased level of PCO compared to the control and Rr.groups insert ignore into journalissuearticles values(p<0.05 and p<0.05); in the kidney tissue. Conclusion: In the study, it was seen that the bioactive substances in Rheum ribes L. insert ignore into journalissuearticles values(root); and quercetin, a standard antioxidant, could be an alternative against the toxic effect of CCl4.
Keywords : carbon tetrachloride, protein carbonyl, quercetin, rheum ribes L, trace elements