Which Phenotypic Method Is the Most Accurate for Detection of Extended – Spectrum β-Lactamases (ESBLs) in Escherichia coli ?

Authors : Montaser My AMRO, Emrah GÜLER, Kaya SÜER, Meryem GÜVENİR

Pages : 624-628

Doi:10.33808/clinexphealthsci.987433

View : 25 | Download : 12

Publication Date : 2022-09-28

Article Type : Research Paper

Abstract :Objective: The aim of the study is to determine the Extended-Spectrum β-Lactamases insert ignore into journalissuearticles values(ESBLs); by three different phenotypic methods of the Escherichia coli insert ignore into journalissuearticles values(E. coli); strains that isolated from various clinical samples. Methods: A total of 93 E. coli samples were isolated from hospitalized patients. Antibiotic susceptibility tests were done by automated system Phoenix 100 insert ignore into journalissuearticles values(Becton Dickinson, Sparks, MD, USA);. ESBL production was tested by double disc synergy test insert ignore into journalissuearticles values(DDST);, combined disc test insert ignore into journalissuearticles values(CDT); and three-dimensional test insert ignore into journalissuearticles values(TDT);. All statistical analyses were done using statistical packages SPSS Demo Ver 22 insert ignore into journalissuearticles values(SPSS Inc. Chicago, IL, USA);. Results: In the investigation of ESBL production among E. coli species, 87 insert ignore into journalissuearticles values(93.5%); strains were ESBL positive by DDST, 73 insert ignore into journalissuearticles values(78.5%); strains were ESBL positive by CDT, 71 insert ignore into journalissuearticles values(76.3%); strains were ESBL positive by TDT. According to statistical analysis: There were statistical differences between DDST-CDT insert ignore into journalissuearticles values(p=<0.001); and DDST-TDT insert ignore into journalissuearticles values(p=<0.001);. However, there was no statistical difference between CDT-TDT insert ignore into journalissuearticles values(p=0.207); Conclusion: According to our study results, DDST test was more advantageous than CDT and TDT such as was not require additional financial expenditure and time, and can be easily used in routine laboratories. Therefore, routine monitoring of ESBL with DDST should be determined because of the conspicuous prevalence of ESBL forming and multidrug-resistant of E. coli.
Keywords : Escherichia coli, ESBL, Enterobacteriaceae, Disc diffusion test