Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats

Authors : Abdulsamet TANIK, Arzum Güler DOGRU, Mehmet GÜL

Pages : 308-315

Doi:10.33808/clinexphealthsci.1080508

View : 36 | Download : 94

Publication Date : 2023-06-15

Article Type : Research Paper

Abstract :Objective: In the present paper, it was purposed to examine the late impacts of bone tissue recoveries in combination with synthetic grafts and Ankaferd Blood Stopper insert ignore into journalissuearticles values(ABS);, which are effective in bone wound healing in diabetic rats. Methods: A total of 64 Wistar albino male rats with diabetes were studied. A bone deformity was generated in the calvarium of diabetic rats. These diabetic rats are divided into 4 different groups. Only saline was applied to the bone defect in group 1, Beta-tricalcium phosphate insert ignore into journalissuearticles values(β-TCP); graft was administered to Group 2, ABS solution to Group 3, insert ignore into journalissuearticles values(β-TCP+ABS); were administered to group 4. Eight rats from each group were sacrificed on the 28th day and another 8 rats on the 56th day. Immunohistochemical, histopathological, and Dual Energy X-ray Absorptiometry insert ignore into journalissuearticles values(DEXA); analyzes of the obtained samples were made. Results: In histopathological measurements, osteoblastic activity and bone regeneration were considerably higher in the group treated with group 2 and group 4 when compared to the control group on the 28th and 56th day insert ignore into journalissuearticles values(p˂ .05);. Western blotting findings showed that the osteopontin insert ignore into journalissuearticles values(OP); and osteonectin insert ignore into journalissuearticles values(ON); expression at 28th day increased dramatically in the treated with group 4. DEXA analyzes revealed that BMC values in groups treated with group 2, group 3 and group 4 on 28th day were considerably higher than the control group insert ignore into journalissuearticles values(p˂ .05);. Conclusion: We can believe and conclude that ABS in combination with a β-TCP bone graft will produce more successful outcomes on wound healing and formation of new bone in diabetic rats.
Keywords : Ankaferd blood stopper, bone regeneration, beta tricalcium phosphate graft, DEXA