DIFFERENT APPROACHES FOR BREAST CANCER: VOLTAGE GATED POTASSIUM CHANNELS AND MICRORNAS

Authors : Çağrı ÖNER, Ertuğrul ÇOLAK, Didem TURGUT COŞAN

Pages : 1-17

Doi:10.1501/commuc_0000000181

View : 17 | Download : 5

Publication Date : 2015-06-01

Article Type : Research Paper

Abstract :Micro RNAs and voltage-gated potassium channels insert ignore into journalissuearticles values(VGPCs); both play critical roles in the development of cancer. We aimed to reveal the diversity of miR-126/126*, which effects angiogenesis and vascular development through the inhibition of VGPCs. In this study, potassium channel inhibitors, including tetraethylammonium insert ignore into journalissuearticles values(5mM);, 4-aminopyridine insert ignore into journalissuearticles values(5mM);, margatoxin insert ignore into journalissuearticles values(1µM); and astemizole insert ignore into journalissuearticles values(2µM);, were applied to MCF-7 and MDA-MB-231 breast cancer cell lines. After totally isolating RNA from the cells, Real-Time Polymerase Chain Reaction was used in order to identify gene expressions. OneWay ANOVA was used for variation analyses, while Tukey HSD and Tamhane were used to assess whether multiple comparisons were statistically significant insert ignore into journalissuearticles values(P < 0.001);. Our results showed an increase in miR-126/126* expressions after the channel inhibition of MCF-7 and MDA-MB-231 cell lines insert ignore into journalissuearticles values(P < 0.001);. miR-126/126* expressions were increased using TEA, 4-AP and astemizole in both cell lines. miR-126/126* expressions were only increased through the use of margatoxin in MCF-7. miR-126/126* may interact with voltage-gated potassium channels. In our study, the inhibition of K channels using K channel blockers resulted in an increase of miR-126/126* expression. Therefore, our data suggested that there could be another perspective between K channels and non-coding RNAs in the development of breast cancer
Keywords : Breast Cancer, miR 126 126, Voltage Gated Potassium Channels