INVESTIGATION OF THE ANTIMICROBIAL ACTIVITY OF GAGEA

Authors : Basma Mansur Abdalrahim BADER, Talip CETER, Barış BANI

Pages : 224-231

Doi:10.1501/commuc_0000000218

View : 17 | Download : 6

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :The main objective of this study is to evaluate the antimicrobial effect of ethanol solution extracts of G. dubia A. Terracc. on nineteen gram positive and negative bacteria insert ignore into journalissuearticles values(Bacillus subtilis, Enterococcus faecium, Enterococcus faecalis, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella infantis, Salmonella typhimurium, Salmonella kentucky, Salmonella enterica, Pseudomonas aeruginosa, Pseudomonas fluorescens); and one fungi insert ignore into journalissuearticles values(Candida albicans);. Different parts insert ignore into journalissuearticles values(flowers, leaves, stems, and roots); of Gagea dubia samples, were extracted with 80% ethanol solution. These extracts were tested in vitro for their antimicrobial activity against 15 microorganism by disk diffusion insert ignore into journalissuearticles values(DD); and minimum inhibitory concentration insert ignore into journalissuearticles values(MIC); and minimum bactericidal concentration insert ignore into journalissuearticles values(MBC); tests. Ethanol extracts showed antimicrobial activity against some bacteria. According to the results of disk diffusion test G. dubia have effect on four bacteria insert ignore into journalissuearticles values(B. subtilis, S. aureus, L. innocua and E. durans); and insert ignore into journalissuearticles values(C. albicans); and didn’t show effect on other microorganisms. The antibacterial effect observed in 10 µL extract of G.dubia against B. subtilis, S. aureus, L.innocua with 7,66; 13 and 9 mm zone diameter respectively and 50 µL extract of G. dubia against E.durans with 11 mm zone diameter. G. dubia extract show antimicrobial activity at three concentration insert ignore into journalissuearticles values(10;50 and 100 µL); against C. albicans between 915,66 mm zone diameter. MIC result for G. dubia determined as 5; 1,25; 10; 5 and 0,15 mg/100 µL against B. subtilis, S. aureus, L.innocua, E.durans and C. albicans respectively. According to MBC test all MIC value against maicroorganism determined as bacteriostatic
Keywords : Gagea dubia, Liliaceae, Antimicrobial effect, MIC, MBC, Disk diffusion