ISOLATION OF ENTOMOPATHOGENIC FUNGI FROM TURKEY SOIL AND TESTING OF DIFFERENT DOSES ON GALLERIA MELLONELLA (LEPIDOPTERA: PYRALIDAE)

Authors : Yusuf DOĞAN, Nevin KESKİN

Pages : 213-242

View : 20 | Download : 7

Publication Date : 2020-12-31

Article Type : Research Paper

Abstract :Isolation of biological control agents which would be used with pests control could be done with different methods. The most sensitive of these methods is the insect bait method. Besides, it is known that the effects of different entomopathogen fungi isolates, which would be used as biological control agents varies in different hosts. In this study, since the target pest organism is the larval period of Galleria mellonella Linnaeus insert ignore into journalissuearticles values(Lepidoptera: Pyralidae);, this pest is used for insect bait method and as a result of isolation from 180 soil samples, 48 Beauveria bassiana Bals.-Criv. Vuill. insert ignore into journalissuearticles values(Hypocreales: Cordycipitaceae); and 1 Beauveria brongniartii insert ignore into journalissuearticles values(Sacc.); Petch insert ignore into journalissuearticles values(Hypocreales: Cordycipitaceae); was detected but Metarhizium spp. wasn’t detected. Furthermore, it is known that the precise determination of the entomopathogen fungi isolates, plays a crucial role in the success of a biological control program. In this study, as biological control agent, four concentrations insert ignore into journalissuearticles values(1x104, 1x105, 1x106 and 1x107 conidiya/ml); of 10 different isolates of Beauveria bassiana Bals.-Criv. Vuill. insert ignore into journalissuearticles values(Hypocreales: Cordycipitaceae);, which were obtained from soils of our country and a standard strain from Denmark were applied to Galleria mellonella Linnaeus insert ignore into journalissuearticles values(Lepidoptera: Pyralidae); larvae. When Lethal times insert ignore into journalissuearticles values(LT50); compared, it was found that the highest concentration of each isolates was also the most effective one.
Keywords : Biological control, Entomoğathogen fungi, Insect bait method, Beauveria bassiana, Galleria mellonella