BIOCHEMICAL AND PATHOLOGICAL EFFECTS ON THE MALE RAT HEPATIC TISSUE AFTER EXPOSURE TO 900MHZ ELECTROMAGNETIC FIELD DURING ADOLESCENT PERIOD

Authors : Ayşe İKİNCİ KELEŞ, Hüseyin Serkan EROL, Tuğçe SAPMAZ, Tolga MERCANTEPE, Gökhan KELEŞ, Burcu BİTERGE SÜT, Ersan ODACİ, Mesut HALICI, Sait POLAT

Pages : 25-46

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Publication Date : 2021-06-30

Article Type : Research Paper

Abstract :The aim of this study was to employ biochemical analysis and histopathological techniques to investigate the effects of the application of a 900-megahertz electromagnetic field during adolescence on the rat liver. 24 male Sprague Dawley rats aged 21 days were randomly divided into electromagnetic field, sham and control groups. No procedure was performed on the control group rats. Electromagnetic field group rats were exposed to a 900-megahertz electromagnetic field in an electromagnetic field application cage insert ignore into journalissuearticles values(1 hours/day, every day for 25 days);. The sham group rats were placed in the same electromagnetic field cage without exposure to electromagnetic field. At the end of the experiments, the livers were removed. The livers were used for histopathological evaluation insert ignore into journalissuearticles values(light, electron and immunofluorescence microscopy); and biochemical analyses. Biochemical analyses revealed increased lipid peroxidation and glutathione values while catalase and superoxide dismutase values were decreased in electromagnetic field group. Electron microscopy evaluations identified necrotic hepatocytes with numerous cytoplasmic and mitochondrial vacuoles in electromagnetic field group sections. We also observed an enlarged endoplasmic reticulum and loss of mitochondrial matrix, in addition to the presence of vacuoles. Also, loss of inner and outer mitochondrial membrane integrity was observed. Light microscopy evaluations revealed sinusoidal capillary dilatation and degeneration in hepatocytes with cytoplasmic swelling, vacuolar degeneration and pyknotic nuclei in electromagnetic field group sections. Deterioration in the integrity of the collagen fibers was also present around the vena centralis. Additionally, electromagnetic field had no effect on body weight. Therefore, we conclude that continuous 900-megahertz electromagnetic field treatment may cause changes in oxidative stress biomarkers and the morphology of the adolescent rat liver.
Keywords : Adolescence, Electromagnetic field, Histopathology, Liver, Oxidative stress