Dopamine Detection From PC12 Cells With A Carbon-fiber Microelectrode Controlled By A Hommade System

Authors : Tuğba Akkaş, Mustafa Şen

Pages : 104-109

View : 49 | Download : 37

Publication Date : 2023-12-15

Article Type : Research Paper

Abstract :Dopamine (DA) is a neurotransmitter that is naturally produced in the body. Dopamine provides nerve conduction in the central nervous system and plays an important role in the regulation and control of movements, motivation and cognitive development.. Imbalance in the amount of DA can cause many diseases ranging from Parkinson\'s disease to depression. In this study, PC12 cells were chemically stimulated with potassium ions and local electrochemical measurement of DA released by the cells was performed.First, the production of needle-tipped CFMDE was carried out by micropulling method. Carbon-fiber microdisk electrodes are frequently used for effective and high sensitivity detection of dopamine. Afterwards, cell clusters of PC12 cells were obtained and the change in dopamine amount in PC12 cell clusters was measurement. For the measurement process, a microcontroller unit integrated to a inverted microscope was used to precisely position the needle-tipped electrodes. DA release was successfully measured as a result of chemical stimulation with K+ ions. Basically, a homemade system was developed to analyze DA release triggered by chemical or physical stimuli.
Keywords : Karbon fiber Mikroelektrot, Dopamin, PC12 hücreleri, Elektrokimyasal Tespit, Lokal Analiz