Protective Effects of Crocin on Experimental Gastrocnemius Muscle Ischemia/Reperfusion Model in Rat

Authors : Betul APAYDIN YILDIRIM, Selcuk ALBAYRAK

Pages : 116-120

View : 80 | Download : 8

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :The aim of the present study was to define whether Crocin, has a regulatory effect againstexperimental gastrocnemius muscle ischemia/reperfusion injury model in rats.A total of 32 Sprague-Dawley male, 200-250g rats were randomly divided into four groups: Control, Crocin insert ignore into journalissuearticles values(60mg/kg/i.p);, I/R and I/R+Crocin groups. All rats were anesthetized with xylazine and ketamine. Except for the control and Crocin group, the left lower limbs of the other 2 groups were applied to 2 hours of ischemia and 2 hours of reperfusion with tourniquet. SOD, MDA, GSH, CAT, GPx, MPO, NO, IMA, AOPP ve PON levels were measured in the plasma; the levels of SOD, MDA, GSH, CAT and GPx were determined in gastrocnemius muscle. Increases in SOD, GSH, CAT, GPx, AOPP and PON levels, decreases in the MDA, MPO, NO and IMA levels were significant in the Crocin groups according to the I/R group. These differences were statistically significant. The results of this study support the possibility that Crocin may play a protective role against skeletal muscle injury caused by I/R in rats by reducing oxidative stress. Crocin can be safely used for ischemia/reperfusion injury.  
Keywords : Crocin, Ischemia, Rat, Oxidative stress, Reperfusion

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