Maturation, Fertilization and Implantation of Cattle Ova Cultured in Different Media

Authors : Saif ALHAFEDH, Thair MOHAMMED, İhasan ALTAMIMI

Pages : 181-188

View : 20 | Download : 7

Publication Date : 2018-12-01

Article Type : Research Paper

Abstract :Several experiments have been conducted to study factors affecting the maturation and fertilization of the cattle ova in the laboratory. Ovaries were collected from 77 cow reproductive systems in Al- Shuala Massacre- Baghdad. Oocytes have been obtained from these samples by OCRC method and finishing. Oocytes were classified into several grades from both methods, and then oocytes class A and B only were chosen for maturation, where oocytes were incubated in multiple media including; TCM-199, MEM, SOF, SOF+BCS, SOF+BSA in incubator 5% CO2, 90% humidity at 38.5ºC temperature for 24 hours. Semen has been obtained by extracting of the epididymal tail, and then collected by using a sterile 5 ml syringe containing MEM media. Sperms were incubated with mature oocytes at a concentration of 1 × 10 6/ml of the cultivated media in the incubator for 30 hours for the fertilization and to obtain on zygotes. Zygotes were then isolated in the growing media. The developing embryos were monitored every 24 hours and up to 9 days until the reach blastocyst stage.  Results indicate the possibility of obtaining embryos in vitro fertilization in an average 6.31%  insert ignore into journalissuearticles values(111/1604);, and the best media was by via using SOF + BCS media. The study also determined appropriate technologies to enhance in vitro fertilization. ORCS method showed a positive effect on increasing the rate of embryos production in vitro.
Keywords : Slicing, ORCS, TCM 199, MEM, SOF, In vitro fertilization