First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo

Authors : İlktan BAŞTAN, Fırat KORKMAZ, Derya ŞAHİN, Seher ŞİMŞEK, Muharrem SATILMIŞ

Pages : 60-65

Doi:10.46897/livestockstudies.610203

View : 44 | Download : 7

Publication Date : 2021-12-23

Article Type : Research Paper

Abstract :Conventional buffalo semen freezing studies are limited in Anatolian buffaloes, which are overly sensitive to exogenous stimulation. The present study`s object was to determine the main features of Anatolian buffalo semen obtained by artificial vagina method for the first time. A total number of 150 ejaculates were collected from three Anatolian Buffalo bulls insert ignore into journalissuearticles values(app. 4 years of age);. The mean pH, volume and concentration of semen were found 6.63±0.15, 1.61±0.5 ml, 1629±222.67 x106 spermatozoa/ml, respectively. The sperm motion characteristics were determined by using a computer-assisted sperm analysis system insert ignore into journalissuearticles values(CASA);; the total and progressively motile sperm values were 57.12±5.63%, 23.22±4.47% and other kinetic parameters such VAP, VSL, VCL, ALH, BCF, STR, LIN were found 94.71±8.48 µm/s, 72.6±7.08 µm/s, 160.9±15.66 µm/s, 7.8±3.75 µm, 29.15±1.56 Hz, 76.91±3.87%, 46.21±2.61%, respectively after thawing. Among buffalo bulls, differences in semen pH values were statistically significant insert ignore into journalissuearticles values(P>0.05);, while differences in ejaculate volume, semen concentration, total motility, progressive motility, VAP, VSL, VCL, ALH, BCF, STR, and LIN were not insert ignore into journalissuearticles values(P>0.05);. As a result, frozen Anatolian buffalo semen can be obtained economically and can be used for animal breeding in assisted reproductive biotechnology such as artificial insemination or in vitro embryo production commercially.
Keywords : Artificial insemination, Bubalus bubalis, CASA, Frozen semen, Mediterranean buffalo

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