Determination of Triploidy in Rainbow Trout, Oncorhynchus mykiss using Erythrocyte Measurements

Authors : Osman Nezih KENANOĞLU, Sevdan YILMAZ, Nergiz SOYTAŞ, Sebahattin ERGÜN, Cüneyt AKI, Fatih TAPAN

Pages : 17-19

View : 25 | Download : 8

Publication Date : 2012-12-20

Article Type : Research Paper

Abstract :In this study, effects of thermal shock insert ignore into journalissuearticles values(26°C); on Oncorhynchus mykiss egg at different duration time insert ignore into journalissuearticles values(5, 10, 15 and 20 min.); to triploid rate was determined. Level of triploid induction for each treatment was evaluated by erythrocyte measurements. For this purpose, the caudal peduncles of 40fry for each group were cut off and blood smears prepared. The cell and nucleus lengths insert ignore into journalissuearticles values(major and minor axis); of 30 erythrocytes for each individual were determined with the aid of a microscope camera insert ignore into journalissuearticles values(with ScopImage 9.0®); and using a micrometer. There was considerable variation in major axisof erythrocytes cell and nucleus both within individual fish and among individuals of a given ploidy. In the major axis length of erythrocyte, cell and nucleus of 10, 15, 20 min thermal shocked groups were statistically significant compared to the control groups insert ignore into journalissuearticles values(p<0.05);. The presented study demonstrated that the increases of triploid yields are correlated to length oferythrocyte major axis. In conclusion, the best triploid yield for O. mykiss was at 26°C for 15 min initiated 15 min after fertilization.
Keywords : Rainbow trout Oncorhynchus mykiss, Heat shock, Triploid 3n, Diploid 2n, Erythrocyte measurements