Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity

Authors : Muammer KIRICI, Muhammed ATAMANALP, Mahinur KIRICI, Şükrü BEYDEMİR

Pages : 92-101

Doi:10.33714/masteb.709377

View : 54 | Download : 8

Publication Date : 2020-12-31

Article Type : Research Paper

Abstract :In this study, the in vitro effects of some metal ions insert ignore into journalissuearticles values(Ag + , Cd 2+ , Cu 2+ , Fe 2+ , Ni 2 + and Pb 2+ ); on freshwater fish Capoeta umbla liver and gill glucose 6-phosphate dehydrogenase insert ignore into journalissuearticles values(G6PD); have been investigated. For this purpose, C. umbla liver and gill G6PD enzymes were purified, with a specific activity of 31.52 and 22.83 EU/mg protein, 3353.2 and 736.5 fold in a yield of 19.28% and 23.15%, respectively. In order to control the enzyme purification SDS-PAGE showed a single band for the enzyme. In addition, in vitro effects of metal ions on enzyme activity were researched. As a result, Ag + , Cd 2+ , Cu 2+ , Ni 2+ and Pb 2+ inhibited fish liver G6PD; Ag + , Cd 2+ , Fe 2+ , Ni 2 + and Pb 2 + inhibited fish gill G6PD. Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag + . Our results also demonstrate that these metals might be dangerous at low micromolar concentrations for fish G6PD enzyme.
Keywords : Glucose 6 phosphate dehydrogenase, Capoeta umbla, Enzyme purification, Inhibition, Metal ions, Liver

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