Utilization of Polyimide Materials for Enhanced Electrochemical Detection of Cholesterol

Authors : Fatma Bilge Emre

Pages : 41-46

Doi:10.46572/naturengs.1488623

View : 42 | Download : 54

Publication Date : 2024-06-29

Article Type : Research Paper

Abstract :The objective of this study was to utilise a range of polyimide materials for the determination of cholesterol. Cholesterol is a waxy, fat-like substance produced by the liver and is essential for the construction of cells and the production of vitamins and other hormones. Blood cholesterol levels are an important parameter in the diagnosis and treatment of a number of clinical diseases, including arteriosclerosis, cardiovascular ailments, hypertension and others. Cholesterol levels in the blood were measured using a number of chemical and enzymatic methods. Electrochemical tests were conducted using Cyclic Voltammetry (CV) and Time Base (TB) techniques. The surface morphologies of the modified electrode surfaces were examined in detail using scanning electron microscopy (SEM) techniques. In the enzymatic reaction, hydrogen peroxide was formed as a result of the enzymatic reaction between cholesterol and cholesterol oxidase, which was measured at 0.650–0.750 V against Ag/AgCl. In this study, poly[1,3,5-tri(aminophenyl)hegzahydro-1,3,5-pyromellithimide] and poly[2,4,6-triaminopirimidyn benzophenondiimide] were employed as polymeric materials to immobilise cholesterol oxidase. It was hypothesised that these two polyimides could be utilised as an appropriate medium for the immobilisation of cholesterol oxidase.
Keywords : polyimide, cholesterol oxidase, polymer matrix, biosensor