Optimization of Graphite-Mineral Oil Ratio With Response Surface Methodology in Glucose Oxidase Based Carbon Paste Electrode Design

Authors : Esra YAĞIZ, Gül OZYİLMAZ, Ali Tuncay OZYİLMAZ

Pages : 22-33

Doi:10.28978/nesciences.1098655

View : 19 | Download : 9

Publication Date : 2022-04-04

Article Type : Research Paper

Abstract :In this study, construction of amperometric glucose biosensor was carried out by immobilizing of glucose oxidase insert ignore into journalissuearticles values(GOD); on carbon paste electrode insert ignore into journalissuearticles values(CPE); which was coated with polyaniline insert ignore into journalissuearticles values(PANI); by cyclic voltammetry technique. Since the current values to be measured depending on the glucose concentration will be affected by the graphite:mineral oil composition of the electrode, this parametres were optimized by response surface methodology insert ignore into journalissuearticles values(RSM);. For this, State Ease Design Expert 8.0.7.1. insert ignore into journalissuearticles values(Serial Number: 0021-6578); software software was used applying Optimal Design. By using data obtained, Design Expert software suggested quadratic model to predict current values in terms of working parameters. In study, experimentally measured current values and predicted values by model were considerably found compatable and suitability of model was supported by ANOVA test.
Keywords : Carbon paste electrode, Response Surface Methodology, Glucose Oxidase, biosensor, polyaniline