In Vitro Micropropagation of Pogostemon erectus (Dalzell) Kuntze in Liquid Culture Medium

Authors : Muhammet DOGAN

Pages : 80-88

Doi:10.28978/nesciences.1098681

View : 24 | Download : 8

Publication Date : 2022-04-04

Article Type : Research Paper

Abstract :Pogostemon erectus insert ignore into journalissuearticles values(Dalzell); Kuntze is a valuable aquatic-ornamental plant. This study was designed for the multiplication of P. erectus in a liquid culture medium. In this context, shoot tip explants of P. erectus were placed in Murashige and Skoog insert ignore into journalissuearticles values(MS); food solutions including Zeatin insert ignore into journalissuearticles values(ZEA: 0.1-2.4 mg/L); and indole acetic acid insert ignore into journalissuearticles values(IAA: 1.2 mg/L);. In addition, experiments were set up in hormone-free environments as controls. In general, high-frequency results were recorded. 100 % regeneration was determined in the treatment of 0.6 mg/L ZEA + 1.2 mg/L IAA. The best results for shoot count and shoot length were obtained with 0.6 mg/L ZEA + 1.2 mg/L IAA and 0.3 mg/L ZEA + 1.2 mg/L IAA, respectively. Generally, low regeneration values were seen in nutrient solutions using high or low doses of ZEA. Shoots were rooted in MS nutrient media with 0.25 mg/L IAA. Then they were transferred to an aquarium and successfully adapted to external conditions.
Keywords : MS medium, Shoot regeneration, Shoot tip explant, Tissue culture