Design of siRNAs Against Immune-Implicated Atherosclerosis Genes: Computational Study

Authors : Haitham Almadhagi

Pages : 12-18

Doi:10.33435/tcandtc.1246320

View : 79 | Download : 180

Publication Date : 2024-05-21

Article Type : Research Paper

Abstract :Atherosclerosis is a chronic, immune-implicated, disease with high numbers of mortality globally. The aim of the current study is to target these genes by specific siRNA utilizing bioinformatics tools. Eight siRNAs were designed via RNAxs from C1QA and ITBG2 gene sequences retrieved from NCBI database. GC% and Tm of siRNAs were calculated through OligoCalc web interface. In addition, hybridization energy of siRNAs with the corresponding target sequences as well as docking to argonaute 2 protein were performed using DuplexFold and HDock. The designed siRNAs exhibited acceptable GC content and Tm values. Besides, the hybridization energy and docking scores were highly significant to block the expression of the mentioned genes. In conclusion, the designed siRNAs are superior candidates for silencing immune-mediated atherosclerotic genes which deserve further consideration.
Keywords : Atherosclerosis, siRNA, gene silencing, RNA interference, docking