Bromophenol Blue Removal by Pseudochloris wilhelmii and Acute Toxicity on Daphnia magna

Authors : Elif Betül Kağızman, Şeyda Fikirdesici Ergen, Burcu Ertit Taştan

Pages : 456-465

Doi:10.35193/bseufbd.1663157

View : 77 | Download : 141

Publication Date : 2025-11-30

Article Type : Research Paper

Abstract :The use of dyes is increasing in different industrial areas and this causes environmental problems. Although dyes are removed from wastewater with many different physical and chemical treatment techniques, there is a need for environmentally friendly removal technologies. Although Pseudochloris wilhelmii used in many biotechnological studies, bromophenol blue removal and toxicity have not been investigated yet. Furthermore, there are almost no studies on the bioremoval and toxicity of bromophenol blue dye. In this study bromophenol blue bioremoval and toxicity was tested on P. wilhelmii and Daphnia magna. The maximum bioremoval was obtained at 5.04 mg/L dye concentration as 50.04% at 72 hours. The chl (a+b) concentration was recorded as 0.196 µg/mL and dye removal was 5.66% at the highest dye concentration studied 76.26 mg/L. The increasing bromophenol blue concentration did not completely inhibit the microalgal growth. The effects of bromophenol exposure on D. magna were examined at 24, 48, and 72 hours, and it was observed that the acute toxicity effects increased with exposure time. The highest acute toxicity effect was determined at 72 hours, with a lethal concentration of 129.038 mg/L for D. magna. As a result of the study, it was concluded that P. wilhelmii has the potential to be used for bromophenol blue treatment and this dye may be toxic to aquatic organisms at high concentrations.
Keywords : Akut toksisite, Bromofenol mavisi, Daphnia magna, Pseudochloris wilhelmii

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