Combinations of essential oils obtained from medicinal plants with L. angustifolia: Determination of their antimicrobial effects

Authors : Derya Doğanay, Şevval Maral Özcan Aykol, G. Vildan Seyhan, İlker Demirbolat, Esra Mertoğlu

Pages : 175-185

Doi:10.53445/batd.1789276

View : 116 | Download : 95

Publication Date : 2025-12-30

Article Type : Research Paper

Abstract :In this study, the antimicrobial effects of essential oils obtained from 7 different plants (Lavandula angustifolia, Lavandula intermedia, Cupressus sempervirens, Syzygium aromaticum, Salvia officinalis, Allium sativum, Pimpinella anisum) and their combinations with Lavandula angustifolia, were investigated against 11 pathogenic bacteria (Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 29213, Staphylococcus haemolyticus ATCC 43252, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603, Escherichia coli ATCC NRRLB 3704, Acinetobacter baumannii ATCC 19606, Bacillus subtilis ATCC 6633, Proteus vulgaris ATCC 13315, Enterococcus faecalis ATCC 29212, Aeromonas hydrophila ATCC 95080). The synergistic effect of essential oils and their combinations with L. angustifolia were determined using the agar well diffusion method. Each essential oil was subjected to gas chromatography analysis in order to identify their chemical compositions. According to the results, generally all essential oils exhibited the greatest inhibition zones for Gram-positive bacteria than for Gram-negative bacteria. Among the essential oil combinations, the highest synergistic effect (166.7%) was obtained in L. angustifolia-P.anisum combination against P. aeruginosa ATCC 27853. Additionally, the L. angustifolia-S. aromaticum combination was observed to exhibit a synergistic effect on 5 bacteria (K. pneumoniae, E. coli, E. faecalis, S. haemolyticus and A. hydrophila). These findings suggest that L. angustifolia combined with specific essential oils particularly L. intermedia and S. aromaticum may serve as promising natural antimicrobial agents, offering enhanced inhibitory activity against clinically important bacterial pathogens.
Keywords : Uçucu yağlar, antibakteriyel aktivite, agar kuyu difüzyon yöntemi, sinerjik etki, patojen bakteri, inhibisyon bölgesi

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