Formaldehit Toksisitesine Karşı Yeşil Çay Özütünün Koruyucu Etkisi: Swiss Albino Farelerde Genotoksik Değerlendirme

Authors : Betül TAŞLI, Figen ÇİÇEK, Emine YALÇIN, Güray DEMİRTAŞ, Kültiğin ÇAVUŞOĞLU

Pages : 63-73

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Publication Date : 2015-03-21

Article Type : Other Papers

Abstract :In the present study, protective effect of green tea extract against genotoxic effects of formaldehyde on Swiss albino mice were investigated. For this aim, we used the frequency of micronucleus, chromosomal aberrations, abnormal metaphase number and mitotic index as indicators of toxicity. Animals were randomly divided into six groups of six animals each. Green tea extract was applied with two different doses (50 and 150 mg/kg) and  formaldehyde was treated with the dosage of 200 mg/kg. All mice were sacrificed at the end of treatment period and the related tissues were isolated. Formaldehyde alone-treated mice presented higher frequencies micronucleus, chrosomal aberration and abnormal metaphases compared with the controls; moreover, the mitotic index was lower than in controls. Oral treatment with gren tea extract significantly ameliorated the indices of genotoxicity induced by formaldehyde. Both doses of Green Tea extract tested provided significant protection against formaldehyde-induced toxicity, and its strongest effect was observed at the dose level of 150 mg/kg of body weight. The results obtained from this study suggest that Green Tea is a effective protective agent against Formaldehyde induced toxicity.
Keywords : Formaldehit, Yeşil çay özütü, Kromozomal anormallik Mikronukleus, Mitotik indeks

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