In Vitro Investıgatıon of Cornerstone and GF-677 Rootstocks Tolerance to Drought Stress Conditions

Authors : Rıdvan Parlar, Deniz Eroğul, Nevzat Sevgin

Pages : 151-164

Doi:10.33202/comuagri.1505987

View : 70 | Download : 109

Publication Date : 2025-07-07

Article Type : Research Paper

Abstract :With global warming, the use of drought-resistant rootstocks becomes mandatory. This study evaluated the drought stress response of Cornerstone rootstock under in vitro conditions and compared it with GF-677 rootstock. For this purpose, artificial drought stress was created by adding PEG 8000 at different concentrations to the MS nutrient medium under in vitro conditions, and following the PEG 8000 application, characteristics such as shoot number per explant, shoot length, shoot fresh weight, shoot dry weight, chlorophyll content, leaf turgor weight, relative water content, and proline amount were examined in the rootstocks. As PEG 8000 concentration increased, shoot number and shoot length decreased in both rootstocks. At higher PEG 8000 concentrations, Cornerstone rootstock showed a smaller decrease in chlorophyll content, while both rootstocks exhibited increased proline content. Cornerstone rootstock accumulated higher proline levels than GF-677 rootstock at all PEG 8000 concentrations.Fewer shoots, reduced shoot length, controlled increase in fresh and dry weights, stable dry weight under severe drought, higher proline levels, and minimal chlorophyll decrease indicate the Cornerstone rootstock\\\'s drought resistance. The study shows that both rootstocks exhibit different levels of drought resistance under varying conditions. Cornerstone, a new rootstock for Turkey, is recommended for fruit growing in drought-prone areas.
Keywords : anaç, cornerstone, doku kültürü, GF677, kuraklık

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