Chemical characterization and antiproliferative activity of bee venoms from Türkiye against NSCLC

Authors : Fatma Şengül Bağ, Hüsamettin Vatansev

Pages : 205-214

Doi:10.30569/adiyamansaglik.1727540

View : 101 | Download : 132

Publication Date : 2025-12-31

Article Type : Research Paper

Abstract :Aim: This study compared the chemical composition and cytotoxic effect of bee venom samples from Adıyaman (A1), Bursa (B1) and a purified sample (H1) against A549 non-small cell lung cancer (NSCLC) cells. Materials and Methods: Samples were filtered, lyophilized, and, for H1, further purified. The levels of melittin, PLA2, and apamin were quantified by high-performance liquid chromatography (HPLC). Cytotoxicity was assessed in the A549 and HEK-293 cells using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay after 24 h of treatment, with cisplatin as a positive control. IC₅₀ values were calculated to compare the potency of each sample and assess selectivity toward cancer cells. Results: H1 had the highest melittin content (425.6 mg/L) and strongest cytotoxicity in A549 cells (IC50: 1.964 ± 0.013 µg/mL), outperforming A1, B1 and cisplatin (p<0.0001). Conclusion: H1, which is rich in melittin, showed strong antiproliferative activity on NSCLC cells with relatively low toxicity to normal cells, indicating its potential as a natural anticancer agent.
Keywords : Arı zehiri, Melittin, A549 hücreleri, Sitotoksisite, HPLC analizi

ORIGINAL ARTICLE URL