Chromatographic Determination of Denatonium Benzoate in Colognes

Authors : Emine Kübra İnal

Pages : 1840-1848

Doi:10.29130/dubited.1409022

View : 66 | Download : 78

Publication Date : 2024-10-23

Article Type : Research Paper

Abstract :Denatonium benzoate, commercially known as Bitrex, is an ionic compound containing a quaternary ammonium cation (denatonium) and negatively charged benzoate. It is commonly used as a bittering agent in various alcohol-based products to deter ingestion. The national regulations regarding the production and domestic-foreign trade of ethyl alcohol and methanol states that ethyl alcohols for domestic use should contain 0.8 g denatonium benzoate per 100 L of absolute alcohol. This leads to the necessity of quantitative analysis of denatonium benzoate in alcoholic products. Cologne samples of different brands were analyzed using reversed-phase high performance liquid chromatography (RP-HPLC) coupled with UV/vis detector. Isocratic elution was performed on an ODS column with a mobile phase containing 75% MeOH:25% H2O. Since the proposed method does not require any pre-treatment step, the procedure is simple and rapid. The limit of quantitation was found to be 0.17 mg/L. The linearity of the method was tested in the range 0.26 – 15.75 mg/L with a limit of detection of 0.050 mg/L (signal-to-noise ratio of 3).
Keywords : Denatonyum benzoat, Bitrex, Denatüran, Alkol bazlı el dezenfektanı, Kolonya, HPLC