Evaluation Of Pyrazoline-Bearing Primary Sulfonamides As Potent Inhibitors of Lactoperoxidase

Authors : Nurcan Dedeoğlu, Ayşenur Demir, Cem Yamali, Semra Işık

Pages : 36-43

Doi:10.35414/akufemubid.1699085

View : 74 | Download : 156

Publication Date : 2026-01-19

Article Type : Research Paper

Abstract :Lactoperoxidase (LPO E.C. 1.11.1.7) is a glycoprotein belonging to the class of oxidoreductases and constitutes a notable member of the peroxidase enzyme family. The biological significance of LPO is attributed to its role as a natural defense system against microbial infection. In the current study, to examine the inhibitory effects of 8 different polyfluoro-substituted pyrazoline-type primary sulfonamide derivative compounds on LPO enzyme activity, LPO enzyme was extracted from buffalo milk and purified using Sepharose 4B-etilendiamin-4-tiyoüreido-benzensülfonamid affinity column, resulting in a 2212.45-fold purification, a 19.31 % yield, and a specific activity of 4955.9 EU/mg. Ki values for the active inhibitors were determined to be between 1.02±0.53-6.67±2.94 µM, while their IC50 values were in the range of 0.144±0.03-0.798±0.07 µM. The IC50 value and Ki values for the most effective inhibitor, compound 3, were calculated as 0.144±0.03 and 6.06±4.06 µM, respectively, and it was found that this compound inhibited LPO enzyme activity non-competitively.
Keywords : Laktoperoksidaz, manda sütü, pirazolin, sülfonamid, florin, enzim inhibisyonu

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