Effects of Citrobacter freundii ATP Production and Oxygen Intake Two Different Plasmids Carrying the Vitreoscilla Hemoglobin Gene (vgb)

Authors : Hüseyin KAHRAMAN, Aslı Giray KURT

Pages : 28-33

View : 26 | Download : 12

Publication Date : 2017-04-24

Article Type : Research Paper

Abstract :In this study, the use of recombinant oxygen uptake system on production of ATP production, oxygen uptake and cell mass was investigated. Citrobacter freundii and their vgb recombinants were used. Citrobacter freundii, a broad host range plasmids Cf[pMK 79] and Cf[pUC 8:15] was used. Best ATP production by Cf[pMK 79] (0.17 mg/ g). C f[pMK 7 9] s train t he l evel o f o xygene u ptake w as h igher t han C f[pUC 8 :15]. Whereas Cf[pMK 79] the OD600 values have been falling at the end of 48 hours.
Keywords : Citrobacter freundii, Vitreoscilla hemoglobin gene vgb, ATP production