Investigation of the Usability of Polymeric Nanoparticle-Based Transfection Agent for CRISPR/CAS9 in HUVEC Cell Line

Authors : Rizvan İmamoğlu, Özlem Kaplan, Mehmet Koray Gök, İsa Gökçe

Pages : 712-719

Doi:10.35414/akufemubid.1584459

View : 102 | Download : 105

Publication Date : 2025-08-04

Article Type : Research Paper

Abstract :Genome editing technology is a promising, popular technology for diagnosing and treating many diseases. One of the most popular techniques utilized in genome editing research nowadays is the CRISPR/Cas system. The CRISPR/Cas system holds considerable potential for diagnostic and therapeutic applications due to its ease of use, speed, and affordability. The safe and effective delivery of the CRISPR-based genome editing system to the intended human body cells is one of the system\\\'s problems. This study investigated the silencing of the COX-2 gene via the CRISPR/Cas system using a PβAE-based transfection agent in the HUVEC cell line. Western blot analysis was performed to confirm COX-2 gene silencing in the HUVEC cell line, and the effects of COX-2 gene silencing on cell migration in these cells were examined. As a result, the transfection efficiency of the PβAE-based transfection agent for the HUVEC cell line was determined as 90-95%, and the CRISPR efficiency was calculated as 57.12% based on the Western blot results. It was revealed that the PβAE-based nanoparticles used in this study can be used as a good transfection agent in CRISPR/Cas-mediated genome editing in the HUVEC cell line, especially in plasmid transfection.
Keywords : CRISPR, Genom Düzenleme, HUVEC Hücre Hattı, Transfeksiyon

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