Modeling and Optimizing Homogenizator-Assisted Extraction of Antioxidant Compounds from Linden and Characterization by HPLC-PDA

Authors : Furkan Burak Şen
Pages : 1407-1415
Doi:10.35414/akufemubid.1617161
View : 107 | Download : 157
Publication Date : 2025-12-03
Article Type : Research Paper
Abstract :In this study, the modeling and optimization of homogenizer-assisted extraction (HAE) of antioxidants from linden were carried out using response surface methodology (RSM) within the framework of a face-centered composite design. Experimental factors included homogenization speed, extraction time, solvent-to-solid ratio, and solvent concentration. The HAE process was optimized to maximize the total antioxidant capacity (TAC) which determined by using CUPric Reducing Antioxidant Capacity (CUPRAC) of the linden extract. The models created for the TAC responses demonstrated a significant relationship (p < 0.0001) between the dependent response and the independent parameters. Water ratio was identified as the most significant operational factor in the HAE process, while solvent-to-solid ratio was determined to be the least significant parameter. The experimental data obtained from the model showed strong agreement with the results predicted by the model. This indicates the model\\\'s suitability and success in optimization. Under the optimum operational conditions of HAE, 0.912 mmol TR/g-dried sample was achieved as TAC. Based on the TAC values, the HAE method was found to be much more efficient than the traditional heat extraction method under the same experimental conditions. The individual antioxidants of the linden extract were identified using HPLC on a C18 column with a gradient elution method. Using HPLC–PDA analyses, 12 antioxidans were identified in the linden extract. As a result, the modeled methodology is proposed as an applicable method for the extraction of antioxidants and phenolics from linden in the natural product industry.
Keywords : Ihlamur, Ekstraksiyon, Antioksidan, HPLC-PDA

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