New Aptamer Pairs as a Candidate for the Rapid Detection of Food Pathogen, Salmonella enteritidis

Authors : Dilek Çam Derin

Pages : 1-9

View : 75 | Download : 102

Publication Date : 2024-04-30

Article Type : Research Paper

Abstract :Food pathogens are crucial for human and animal health. S. enteritidis, enteric bacteria, is one of the most popular pathogen and causes salmonellosis. Vomitting, diarrhea, fever and abdominal cramps are typcial symptoms occuring at 12-72 hour after the infection and can cause to death. Food based infections in the USA and EU are primarily caused by S. enteritidis. Thus, early and rapid detection of bacteria is always highly significant. In this sense, lateral flow assays (LFAs) are notably advantageous with regards to the obtaining rapid results and practical use. LFAs are commonly prepared by antibodies and aptamers. Aptamers are single stranded DNA and RNA molecules and bind to the target molecules, selectively. Gold nanoparticles (AuNPs) are the most common labels used for LFAs. In this work, it is aimed to develop the aptamer paired strip assay (APSA) platform by using aptamer pairs specific to S. enteritidis. It was observed that 1st aptamer pairs are suitable for developing the LFAs for the recognition of S. enteritidis compared to the other pairs and M180 membrane was found as suitable membrane in terms of the flow rate and positive results.
Keywords : S enteritidis, Aptamer pairs, Lateral flow assay, Food pathogen